Pulse-Field Analysis Diversity of Extended Spectrum Beta Lactamases Producing Gram Negative Bacteria Isolated from Periplaneta americana (Cockroaches)

Abstract

Extended spectrum beta lactamase (ESBL) producing bacteria are organisms of public health significance that have been implicated in prolonged hospital stay, treatment failures and higher fatalities. These organisms were originally thought to be widely distributed in hospital, but have since grown in terms of their epidemiological coverage. This study was therefore aimed at using pulse-field gel electrophoresis (PFGE) to understand the diversity of ESBL producing organisms originating from cockroaches in Ago-Iwoye, Nigeria. The diversity was determined by using a set of twenty six (26) ESBL producing bacterial strains isolated from cockroaches sampled in Ago-Iwoye community, Ogun State, Nigeria. PFGE analysis revealed three pulsotypes (distinct profile) with no distinct isolates present on a pulsotype. It was however observed that no pulsotype had less than two (2) isolates in any case, while isolates from different communities displayed identical profiles. In conclusion, clonal distributions of ESBL producing organisms on cockroaches are without bias to specimens and/or the community sampled.

Keywords: ESBL, Gram negative bacteria, PFGE, Diversity