Ameliorative Effects of Vitamins C and E on Haematotoxicity and Spleen Histopathology Induced by Dichlorvos Insecticide in Female Wistar Rats

Authors

  • Halima Mfaume Department of Zoology and Wildlife Conservation, College of Natural and Applied Sciences, University of Dar es Salaam, Dar es Salaam, Tanzania
  • Harishchandra Pratap Department of Zoology and Wildlife Conservation, College of Natural and Applied Sciences, University of Dar es Salaam, Dar es Salaam, Tanzania
  • Claudius Luziga Department of Veterinary Anatomy, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, Morogoro, Tanzania
  • Felister Urasa Department of Zoology and Wildlife Conservation, College of Natural and Applied Sciences, University of Dar es Salaam, Dar es Salaam, Tanzania

Abstract

This research aimed to assess the toxicological effects of dichlorvos insecticide (2,2-dichlorovinyl dimethyl phosphate or DDVP) on haematological indices of female Wistar rats and to investigate the antioxidant properties of vitamins C and E in ameliorating haematotoxicity. The rats were divided into 6 groups; control-water, control-oil, dichlorvos group, dichlorvos + vitamin C group, dichlorvos + vitamin E group and dichlorvos + vitamin C + E group. Dichlorvos and vitamins were orally administered to rats with an interval of half an hour between treatments. The control-oil group was given 2 ml corn oil and the control-water group was given water adlib. The treatments were done for 28 consecutive days and blood samples were taken by cardiac puncture. The following haematological parameters were analysed: RBC, WBC, Hb, PCV, MCV, MCH, MCHC and THR. Bone marrow smears were prepared on 14th and 28th days for various blood stem cells evaluation and spleen tissues for histopathology. There was a significant decrease (p Ë‚ 0.01) in RBC counts, PCV, MCV and MCH values, whereas the WBC, THR counts and MCHC values increased significantly (p Ë‚ 0.01) in dichlorvos treated rats as compared to the controls and dichlorvos + vitamins co-treated groups. There were no significant differences in blood parameter counts between controls and dichlorvos + vitamins co-treated groups. In the bone marrow smear of the dichlorvos treated group, there was increased number of megakaryocytes and mature neutrophils. In conclusion, findings from the current study revealed that, vitamins C and E supplements were capable of mitigating the haematotoxic effects induced by dichlorvos insecticide in Wistar rats.

Keywords: Dichlorvos toxicity; Blood parameters; Spleen; Vitamin C; Vitamin E

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Published

2023-06-30

Issue

Vol. 49 No. 2 (2023)

Section

Biological Sciences Section