Cellulase-Producing Bacteria Isolated from MufindiPaper Mill Industrial Effluent, Iringa Tanzania

Authors

  • Erick M. Sinza Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dar es Salaam, P. O. Box 35179, Dar es Salaam, Tanzania.
  • AnethD. Mwakilili Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dar es Salaam, P. O. Box 35179, Dar es Salaam, Tanzania.
  • Cyprian B. Mpinda Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dar es Salaam, P. O. Box 35179, Dar es Salaam, Tanzania.
  • Sylvester L. Lyantagaye Department of Biochemistry and Pharmacology, Mbeya College of Health and Allied Sciences, University of Dar es Salaam, P.O.Box 608, Mbeya, Tanzania.

Abstract

Effluent and effluent-contaminated soil samples collected from Mufindi Paper Mill Limited, Iringa Tanzaniawere investigated for the presence of cellulolytic bacteria. The components of cellulase are endoglucanase, exoglucanase and β-glucosidase and these are very important for complete hydrolysis of cellulose to glucose monomers. Isolates were grown onnutrient agarandscreened for cellulolyticactivityon carboxymethylcellulose (CMC)agar plates usingGram’s iodinesolution. Cellulases activities; endoglucanase, exoglucanase and β-glucosidasewere assayed using CMC, avicel and cellobiose substrates,respectivelyby dinitrosalicyclic acid method. Out of 17 cellulolytic isolates obtained, crude enzyme extracts of only five isolates expressedall cellulasesactivities,the highest activity being on cellobiosefollowed by CMC and least on avicel.The maximum endoglucanase (0.0701±0.010 U/ml), exoglucanase (0.0081±0.001 U/ml) and β-glucosidase (0.0738±0.002 U/ml) activities from submerged fermentation culture wereobserved from isolate 8, 1 and 12,respectively.Based on 16S rRNA sequences analyses,three isolates were identified asPararheinheimeramesophila, Bacillus cereusand Bacillus flexus. The other two isolateswere identified as Bacillus megaterium.

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Published

2025-03-27

Issue

Vol. 47 No. 1 (2021)

Section

Articles